Core Facility Session
Thursday, September, 30th, 2021: 2:30pm – 4:00pm Session 3
How full spectrum cytometry becomes our new normal
Boston University School of Medicine, Department Pathology and Laboratory Medicine and Flow Cytometry Core Facility
Full spectrum cytometry is taking over flow cytometry world, and we are now watching the benefits of this advancement across diverse fields of research that employ cytometric analysis. In this talk, I will discuss what makes spectral cytometry approach different when implemented in a research project, and what staple steps remain same as with ‘conventional’ flow cytometry. This process includes instrument acquisition and calibration, experimental/panel design, acquisition of cytometric readouts, and building a data analysis pipeline. I will bring in several illustrations from the research that is ongoing in our lab including our recent studies focused on lung immunology and hematopoietic stem cell characterization, and will discuss why spectral flow cytometry was indispensable for the success of these projects.
Anna C. Belkina is an Assistant Professor of Pathology and Laboratory Medicine and the Director of the Flow Cytometry Core Facility at Boston University School of Medicine. She received her M.D. degree from Russian State Medical University in Moscow and her Ph.D. degree from Boston University School of Medicine investigating the epigenetic regulation of inflammatory responses driven by bromodomain proteins. Anna’s research is focused on the intersection of immunology and computational biology and her research efforts include investigating the immune landscape of chronic inflammatory diseases and developing computational techniques to assess high-parameter single cell cytometry data. She has designed the opt-SNE algorithm that is now widely used for the visualization of multidimensional cytometry datasets. Over the last several years, Anna has been working on spectral cytometry applications in immunology and stem cell research as well as computational analysis of spectral cytometry data.
Anna is an active member of ISAC (International Society for the Advancement of Cytometry) and has been named 2015-2019 ISAC SRL Emerging Leader. She is a member of the ISAC Council elected for the 2020-2024 term.
Boston University Flow Cytometry Core Facility
Automated optimized parameters for T-distributed stochastic neighbor embedding improve visualization and analysis of large datasets
Multi-Modal Profiling of Human Fetal Liver-Derived Hematopoietic Stem Cells Reveals the Molecular Signature of Engraftment Potential