Wednesday, September, 29th, 2021, 5:00pm – 6:00pm
Imaging Deeper and Faster with Multiphoton Microscop
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
Over the last three decades, multiphoton microscopy has created a renaissance in the imaging community. It has changed how we visualize cells by providing high-resolution, non-invasive imaging capability deep within intact tissue. In this lecture, we will first present the fundamental physics behind multiphoton deep imaging, discuss the challenges of deep tissue, high-resolution optical imaging, and illustrate the requirements and approaches for imaging the dynamic biological function at the cellular level over a large area and depth in awake and behaving animals. We will then present results of deep and fast imaging with long wavelength 3-photon microscopy in the brains and other organs in mouse and fish models. Finally, we will discuss several recent developments, including multi-color 3-photon deep imaging and new laser sources, to further improve the imaging depth and speed in biological tissues.
Chris Xu is the IBM Chair Professor of Engineering at Cornell University. He is the Director of School of Applied and Engineering Physics, the founding co-director of Cornell Neurotech, and the director of Cornell NeuroNex Hub, an NSF funded center for developing and disseminating neurotechnology. Prior to Cornell, he was a member of technical staff at Bell Laboratories and pioneered breakthrough development of fiber optic communication systems based on differential phase-shift keying. He received his Ph.D. in Applied Physics, Cornell University. His current research areas are multiphoton microscopy for deep tissue imaging, multiphoton microendoscopy for clinical applications, and fiber-based devices and systems for optical imaging. He has received three teaching excellence awards and the Cornell Engineering Research Excellence Award. He is a fellow of the Optical Society of America, and a fellow of the National Academy of Inventors.
An adaptive excitation source for high-speed multiphoton microscopy
In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain
In vivo three-photon microscopy of subcortical structures of an intact mouse brain